A method for the determination of extracellular space with 3H inulin.
نویسندگان
چکیده
A method for the extraction and quantitation of 3H inulin from tissues is described. This method does not require either combustion technics or time-consuming acid extraction procedures. Inulin is as soluble in the liquid scintillation medium as it is in water. Therefore, hot-water tissue extracts without further treatment were employed to quantitate l1 inulin. Nearly 100% recovery was obtained when different 3H inulin internal standards were added either to water or to tissue homogenates. The values obtained with this method for the extracellular space of different tissues of the rat agree with those reported in the literature.
منابع مشابه
Polyfructosan-S: A new Inulin-like Substance for the Determination of Glomerular Filtration Rate and Physiologically Active Extracellular Fluid Space in Man
متن کامل
The determination of inulin in blood and in urine by means of vanillin in acid medium.
N IDEAL SUBSTANCE for measuring glomerular filtration must possess certain properties. It must be physiologically inert and nontoxic, so that it does not have any disturbing effect upon the body, particularly upon the kidney. It must not be metabolized to any appreciable degree. It must not dissociate in solution to any great extent, so that the probability of its penetration into the various c...
متن کاملCat Heart Muscle in Vitro
The "osmotic gradient" method, an intracellular microelectrode technique for determining whether an uncharged, water-soluble molecule enters cells or remains extracellular, is described. Using this method, a series of carbohydrates of graded molecular size were examined. In cat papillary o muscles mannitol, molecular radius 4.0 A, remained extracellular while arabinose, molecular radius 3.5 ,~,...
متن کاملDetermination of Extracellular Space in Amphibian Muscle
The volumes of distribution of inulin and dextran in the sartorius, stomach, and cardiac muscle of the frog agree rather closely. That these spaces represent the volume of extracellular water is supported by the observation that efflux of sucrose can be divided into a fast and a slow phase and that the fast-moving fraction corresponds closely with inulin space determined in the same muscle. The...
متن کاملThe effects of different routes of inulin administration on gut microbiota and survival rate of Indian white shrimp post-larvae (Fenneropenaeus indicus)
The present study investigates the effects of different routes of inulin administration as prebiotic on gut microbiota and survival rate of Indian white shrimp post-larvae. Four hundred and fifty Indian white shrimp post-larvae (PL1) were stocked in nine tanks. The tanks were assigned into three treatments: feeding with inulin-treated (110 mg L-1) Artemia nauplii ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 13 11 شماره
صفحات -
تاریخ انتشار 1967