A method for the determination of extracellular space with 3H inulin.

نویسندگان

  • S Addanki
  • F D Cahill
  • J F Sotos
چکیده

A method for the extraction and quantitation of 3H inulin from tissues is described. This method does not require either combustion technics or time-consuming acid extraction procedures. Inulin is as soluble in the liquid scintillation medium as it is in water. Therefore, hot-water tissue extracts without further treatment were employed to quantitate l1 inulin. Nearly 100% recovery was obtained when different 3H inulin internal standards were added either to water or to tissue homogenates. The values obtained with this method for the extracellular space of different tissues of the rat agree with those reported in the literature.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The determination of inulin in blood and in urine by means of vanillin in acid medium.

N IDEAL SUBSTANCE for measuring glomerular filtration must possess certain properties. It must be physiologically inert and nontoxic, so that it does not have any disturbing effect upon the body, particularly upon the kidney. It must not be metabolized to any appreciable degree. It must not dissociate in solution to any great extent, so that the probability of its penetration into the various c...

متن کامل

Cat Heart Muscle in Vitro

The "osmotic gradient" method, an intracellular microelectrode technique for determining whether an uncharged, water-soluble molecule enters cells or remains extracellular, is described. Using this method, a series of carbohydrates of graded molecular size were examined. In cat papillary o muscles mannitol, molecular radius 4.0 A, remained extracellular while arabinose, molecular radius 3.5 ,~,...

متن کامل

Determination of Extracellular Space in Amphibian Muscle

The volumes of distribution of inulin and dextran in the sartorius, stomach, and cardiac muscle of the frog agree rather closely. That these spaces represent the volume of extracellular water is supported by the observation that efflux of sucrose can be divided into a fast and a slow phase and that the fast-moving fraction corresponds closely with inulin space determined in the same muscle. The...

متن کامل

The effects of different routes of inulin administration on gut microbiota and survival rate of Indian white shrimp post-larvae (Fenneropenaeus indicus)

The present study investigates the effects of different routes of inulin administration as prebiotic on gut microbiota and survival rate of Indian white shrimp post-larvae. Four hundred and fifty Indian white shrimp post-larvae (PL1) were stocked in nine tanks. The tanks were assigned into three treatments: feeding with inulin-treated (110 mg L-1) Artemia nauplii ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Clinical chemistry

دوره 13 11  شماره 

صفحات  -

تاریخ انتشار 1967